Single Cell Fluorescence Flow Cytometry. The target protein in the cells is labeled by a fluorophore, and the bound antibody is detected one by one by high-throughput by laser-activated fluorescence.

Single Cell Mass Cytometry (CyTOF). Mass cytometry. Antibody labeling using rare metal isotopes instead of fluorophores breaks through the multiple capacity limits of FFC.

Single Cell Enzyme-Linked Immunospot (ELISpot). The ELISpot assay is a widely used, sensitive method for single-cell cytokine secretion monitoring based on sandwich immunoassay.